These data support the suitability of this mouse model for studying the wound healing response in glaucoma filtration surgery, and as a potentially useful tool for the in vivo evaluation of antifibrotic therapeutics in the eye. Indeed, we verified some of these properties on mouse conjunctival fibroblasts cultured in vitro. We demonstrate that the antiscarring response to mitomycin C is likely to be due to its effects on conjunctival fibroblast proliferation, apoptosis and collagen deposition and the suppression of inflammation. The morphology of the blebs following mitomycin C treatment also resembled well-documented clinical observations, thus confirming the validity and clinical relevance of this model. We show, for the first time, that the mouse model typically scarred within 14 d, but when augmented with mitomycin C, more animals maintained lower intraocular pressures for a longer period of time concomitant with prolonged bleb survival to beyond 28 d. As these models have inherent limitations, we have developed a model of glaucoma filtration surgery in the mouse. Efficacy of these agents has been tested extensively on monkey and rabbit models of glaucoma filtration surgery.

Antiproliferatives such as mitomycin C are commonly used to prevent postoperative scarring. The postoperative subconjunctival scarring response, however, remains the major obstacle to achieving long-term surgical success.

The most common and most modifiable risk factor in glaucoma is elevated intraocular pressure (IOP), which can be managed surgically by filtration surgery. Glaucoma is a progressive optic neuropathy, which, if left untreated, leads to blindness. Seet, Li-Fong Lee, Wing Sum Su, Roseline Finger, Sharon N Crowston, Jonathan G Wong, Tina T Validation of the Glaucoma Filtration Surgical Mouse Model for Antifibrotic Drug Evaluation

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